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didier trono epfl addgene  (Addgene inc)


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    Addgene inc didier trono epfl addgene
    Didier Trono Epfl Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 394 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/didier+trono+epfl+addgene/pCMVR8%2E74+(Plasmid+%2322036)/pm38986614-256-8-11
    Average 96 stars, based on 394 article reviews
    didier trono epfl addgene - by Bioz Stars, 2026-10
    96/100 stars

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    Article Title: Leveraging a self-cleaving peptide for tailored control in proximity labeling proteomics.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HEPES-buffered saline Sigma-Aldrich 51558-50ML Calcium chloride dihydrate Sigma-Aldrich C7902-1KG cOmplete protease inhibitor cocktail Roche 11697498001 Critical commercial assays GoTaq G2 master mix Promega M7822 Gateway LR Clonase II enzyme mix Invitrogen 11791020 Lipofectamine LTX reagent with PLUS reagent Invitrogen A12621 PrimeScript RT kit Takara Bio RR037A Nucleospin mini kit for RNA purification Macherey-Nagel 740955.50 Deposited data Proximity ligation LC-MS/MS raw data and search results for GR This paper PRIDE: PXD046414 Proximity ligation LC-MS/MS raw data and search results for NCAP This paper PRIDE: PXD046413 Proximity ligation LC-MS/MS raw data and search results for NSP7 This paper PRIDE: PXD046412 Experimental models: Cell lines Human: A549 ATCC CCL-185 Human: HEK293T ATCC CRL-3216 Human: HeLa ATCC CCL-2 Human: SK-N-BE(2)-C Laboratory of Frank Speleman (Ghent University) CRL-2268 Human: SHEP Laboratory of Frank Speleman (Ghent University) CVCL_0524 Oligonucleotides SDHA_Fwd This paper TGGGAACAAGAGGGCATCTG SDHA_Rev This paper CCACCACTGCATCAAATTCATG YWHAZ_Fwd This paper ACTTTGGTACATTGTGGCTTCAA YWHAZ_Rev This paper CCGCCAGGACAAACCAGTAT UBC_Fwd This paper ATTTGGGTCGCGGTTCTTG UBC_Rev This paper TGCCTTGACATTCTCGATGGT TSC22D3_Fwd This paper GCGTGAGAACACCCTGTTGA TSC22D3_Rev This paper TCAGACAGGACTGGAACTTCTCC DUSP1_Fwd This paper CTGCCTTGATCAACGTCTCA DUSP1_Rev This paper CTGTGCCTTGTGGTTGTCCT Recombinant DNA pLV-EF1a-tTS/rtTA_PGK-Hygro VectorBuilder N/A pPB-TRE-TurboID-T2A-GR_PGK-BlastR This paper Addgene #218910 pPB-TRE-TurboID-MUTT2AGR_PGK-BlastR This paper Addgene #218911 pPB-TRE-TurboID-T2ANCAP_PGK-BlastR This paper Addgene #218912 pPB-TRE-TurboID-MUTT2ANCAP_PGK-BlastR This paper Addgene #218913 pPB-TRE-TurboID-T2A-NSP7_PGK-BlastR This paper Addgene #218914 pPB-TRE-TurboID-MUTT2ANSP7_PGK-BlastR This paper Addgene #218915 pLV-EF1a-msfGFP-T2A-mCherryNLS_PGK-PuroR This paper Addgene #218916 (Continued on next page) Cell Reports Methods 4, 100818, July 15, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER pCMV-dR8.74 Laboratory of Didier Trono (EPFL) Addgene #22036 pMD2.G Laboratory of Didier Trono (EPFL) Addgene #12259 pCMV-hyPBase Yusa et al.52 Wellcome Trust Sanger Institute Golden Gate assembly kit for T2A split/link proximity labeling proteomics This paper Addgene kit #1000000249 Software and algorithms R (v4.4.0) R Core Team (2022) https://www.R-project.org/ R package: limma Ritchie et al.59 https://doi.org/10.1093/nar/gkv007 R package: imputeLCMD Lazar et al. https://CRAN.R-project.org/ package=imputeLCMD R package: clusterProfiler Wu et al.55 https://doi.org/10.1016/j.xinn.2021.100141 R package: HPAanalyze Tran et al.56 https://doi.org/10.1186/s12859- 019-3059-z R package: ggplot2 Wickham et al. https://ggplot2.tidyverse.org MaxQuant v2.3.4.0 Tyanova et al.60 https://www.maxquant.org/ ZEN 2.3 Lite ZEISS N/A Fiji Schindelin et al. https://doi.org/10.1038/nmeth.2019 qBase+ CellCarta https://cellcarta.com/genomic-data- analysis/ BioRender BioRender N/A Other Streptavidin Sepharose High Performance Resin Cytiva 90100484 OPEN ACCESS ..

    Software:

    Article Title: Leveraging a self-cleaving peptide for tailored control in proximity labeling proteomics.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HEPES-buffered saline Sigma-Aldrich 51558-50ML Calcium chloride dihydrate Sigma-Aldrich C7902-1KG cOmplete protease inhibitor cocktail Roche 11697498001 Critical commercial assays GoTaq G2 master mix Promega M7822 Gateway LR Clonase II enzyme mix Invitrogen 11791020 Lipofectamine LTX reagent with PLUS reagent Invitrogen A12621 PrimeScript RT kit Takara Bio RR037A Nucleospin mini kit for RNA purification Macherey-Nagel 740955.50 Deposited data Proximity ligation LC-MS/MS raw data and search results for GR This paper PRIDE: PXD046414 Proximity ligation LC-MS/MS raw data and search results for NCAP This paper PRIDE: PXD046413 Proximity ligation LC-MS/MS raw data and search results for NSP7 This paper PRIDE: PXD046412 Experimental models: Cell lines Human: A549 ATCC CCL-185 Human: HEK293T ATCC CRL-3216 Human: HeLa ATCC CCL-2 Human: SK-N-BE(2)-C Laboratory of Frank Speleman (Ghent University) CRL-2268 Human: SHEP Laboratory of Frank Speleman (Ghent University) CVCL_0524 Oligonucleotides SDHA_Fwd This paper TGGGAACAAGAGGGCATCTG SDHA_Rev This paper CCACCACTGCATCAAATTCATG YWHAZ_Fwd This paper ACTTTGGTACATTGTGGCTTCAA YWHAZ_Rev This paper CCGCCAGGACAAACCAGTAT UBC_Fwd This paper ATTTGGGTCGCGGTTCTTG UBC_Rev This paper TGCCTTGACATTCTCGATGGT TSC22D3_Fwd This paper GCGTGAGAACACCCTGTTGA TSC22D3_Rev This paper TCAGACAGGACTGGAACTTCTCC DUSP1_Fwd This paper CTGCCTTGATCAACGTCTCA DUSP1_Rev This paper CTGTGCCTTGTGGTTGTCCT Recombinant DNA pLV-EF1a-tTS/rtTA_PGK-Hygro VectorBuilder N/A pPB-TRE-TurboID-T2A-GR_PGK-BlastR This paper Addgene #218910 pPB-TRE-TurboID-MUTT2AGR_PGK-BlastR This paper Addgene #218911 pPB-TRE-TurboID-T2ANCAP_PGK-BlastR This paper Addgene #218912 pPB-TRE-TurboID-MUTT2ANCAP_PGK-BlastR This paper Addgene #218913 pPB-TRE-TurboID-T2A-NSP7_PGK-BlastR This paper Addgene #218914 pPB-TRE-TurboID-MUTT2ANSP7_PGK-BlastR This paper Addgene #218915 pLV-EF1a-msfGFP-T2A-mCherryNLS_PGK-PuroR This paper Addgene #218916 (Continued on next page) Cell Reports Methods 4, 100818, July 15, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER pCMV-dR8.74 Laboratory of Didier Trono (EPFL) Addgene #22036 pMD2.G Laboratory of Didier Trono (EPFL) Addgene #12259 pCMV-hyPBase Yusa et al.52 Wellcome Trust Sanger Institute Golden Gate assembly kit for T2A split/link proximity labeling proteomics This paper Addgene kit #1000000249 Software and algorithms R (v4.4.0) R Core Team (2022) https://www.R-project.org/ R package: limma Ritchie et al.59 https://doi.org/10.1093/nar/gkv007 R package: imputeLCMD Lazar et al. https://CRAN.R-project.org/ package=imputeLCMD R package: clusterProfiler Wu et al.55 https://doi.org/10.1016/j.xinn.2021.100141 R package: HPAanalyze Tran et al.56 https://doi.org/10.1186/s12859- 019-3059-z R package: ggplot2 Wickham et al. https://ggplot2.tidyverse.org MaxQuant v2.3.4.0 Tyanova et al.60 https://www.maxquant.org/ ZEN 2.3 Lite ZEISS N/A Fiji Schindelin et al. https://doi.org/10.1038/nmeth.2019 qBase+ CellCarta https://cellcarta.com/genomic-data- analysis/ BioRender BioRender N/A Other Streptavidin Sepharose High Performance Resin Cytiva 90100484 OPEN ACCESS ..



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